Fisher science education microscope imagej software

Numerous studies have shown that cotransplantation of autologous adipose tissue with ADSC, which are capable of paracrine secretion of various cytokines to promote early vascularization, significantly improves the survival rate of adipose grafts. Therefore, enhancing hematologic reconstruction in the early stages of fat grafting is one of the key factors to promote graft survival.ДЄdipose-derived stem cell (ADSC) is a type of stem cell derived from adipose tissue with multidirectional differentiation potential, which is widely used in the field of regenerative medicine in recent years because of its easy extraction and expansion and strong tissue repair activity. found that grafted fat mostly showed hematologic reconstruction only in the peripheral area, while the central area showed fat necrosis and resorption due to insufficient blood supply and a decrease in grafted tissue volume. Adipose tissue has poor tolerant to ischemia, liposuction will result in the destruction of their original blood supply, and the transplanted adipose tissue needs to rebuild blood supply at an early stage in order to survive stably. However, the unstable retention of fat grafts has been a key clinical problem to be overcome. IntroductionДЄutologous fat grafting is widely used for various treatments in plastic surgery, such as depressed scarring, hemifacial atrophy, and facial rejuvenation, due to its good biocompatibility, low tissue rejection rate, and allergic reaction rate.

In summary, our results provide a theoretical basis for the clinical translation of hADSC-Exo in fat grafting. The results showed that hADSC-Exo can significantly promote grafts angiogenesis and adipogenic differentiation of ADSC to improve the retention of fat grafts and may downregulate the Wnt/ ОІ-catenin signaling pathway to promote the adipogenic differentiation. In this study, we applied hADSC-Exo and HFF-Exo to adipose grafts and explored the potential regeneration mechanism through various means such as bioinformatics, immunofluorescence, immunohistochemistry, and adipogenic differentiation. Therefore, the ability of hADSC-Exo to improve the retention of adipose grafts and its potential regenerative mechanism aroused our strong interest. Our previous study has found that comparing to human foreskin fibroblast exosome (HFF-Exo), human adipose-derived stem cells exosome (hADSC-Exo) can significantly improve the proliferation of vascular endothelial cells and the angiogenic effect of artificial dermal preconstructed flaps. Adipose tissue has poor tolerant to ischemia, so the transplanted adipose tissue needs to rebuild blood supply at an early stage in order to survive stably. Autologous fat grafting has been widely used in plastic surgery in recent years, but the unstable retention of fat graft has always been a key clinical problem.

Fisher science education microscope imagej software